FastQCFastQC Report
Mon 27 Feb 2023
SRR3128425.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128425.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2040858
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT458192.2450851553611275No Hit
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT36940.1810023039329537No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT32850.16096171316181723No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCT30570.14978994128939888No Hit
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG26160.1281813825361686TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC21720.10642582678461705Illumina Single End Adapter 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT152500.087.819131
ACGGGTA2400.078.3245544
ATAGGGC13500.073.451033
ATAGGGA18400.070.7475053
TAGGGTC5700.070.0798654
TAGGGCA9150.069.336494
GAGGGAT22550.068.981184
ATAGCGG4450.067.677471
TAAGGGA14300.067.370073
GTACGGG4750.067.306352
AGGGATG27600.066.746145
ATGAGGG30100.066.696362
AAGGGGC58650.066.505764
AATAGGG27400.066.061882
GATAGGG28000.065.821652
AGTAGGG34050.065.7250062
AAGAGGG66950.065.660132
ATGCGGG11100.065.652232
ATAAGGG27700.065.3464052
TATAGGG16350.065.27532
Produced by FastQC (version 0.11.5)