FastQCFastQC Report
Mon 27 Feb 2023
SRR3128424.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128424.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2034909
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT442702.1755272594499315No Hit
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT40800.20050036635544882No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT36250.1781406441270838No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCT34310.16860704827586886No Hit
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG30340.14909757635353718TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC24340.11961222836008882Illumina Single End Adapter 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT139900.086.83761
ACGGGTA2750.076.904194
ATAGGGC13150.075.407783
TAGACGG3150.071.6690751
TAGGGCA9450.071.117164
TATAGGG14400.070.820132
TCCGTAC6850.070.665292
ATAGAGG12650.069.898591
ATAAGGG23300.068.174232
CCGTACA7000.067.80833
GAATAGG12000.067.805661
AGTAGGG32600.067.754752
ATAGGGA17250.067.565053
AAGAGGG68800.067.28332
AGGGCAT15200.066.476025
TAGAGGG34450.066.435352
GTAGGGA14800.066.365763
GATAGGG28000.066.1298752
ATGAGGG29000.066.118292
AGAGGGC31600.065.139813
Produced by FastQC (version 0.11.5)