FastQCFastQC Report
Mon 27 Feb 2023
SRR3128423.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128423.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2279559
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530992.3293540548851777No Hit
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCT44400.19477451559709577No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTT40190.17630603112268645No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCT37080.16266304140406104No Hit
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG31880.13985161164944623TruSeq Adapter, Index 22 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC25040.10984580789529905Illumina Single End Adapter 2 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT166950.088.174281
TAGGGCA9400.073.9976964
ATAGGGC14300.072.962773
GAATAGG12900.072.939551
ACGGGTA2850.070.910084
AGGGATG30050.069.44215
ATAGCGG6100.069.412151
AGATAGG13350.068.718891
TAGAGGG39600.068.3705142
AGTAGGG34250.067.79662
TAGGGTC6600.067.6494144
GGTAAGG14150.066.828611
AAGGGGC62750.066.659294
CGTAGGG7350.065.8705752
AATAGGG31300.065.626412
AAGAGGG73750.064.882462
GTAGGGC9500.064.808513
AGGGAAT21500.064.704965
TAAGGGA15700.064.361063
GTAGGGT8400.064.343243
Produced by FastQC (version 0.11.5)