FastQCFastQC Report
Mon 27 Feb 2023
SRR3128422.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128422.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1933804
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152950.7909281395632649No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG32740.16930361091403265No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG31770.1642875906762009No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC31400.16237426336898672No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACGAGTCT30950.16004724367102355No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA23030.11909169698687146No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT56550.080.978481
CCGTACA15600.072.9039153
ACGGGTA2300.071.5155264
TAAGCGG3850.070.9016951
GGTAAGG10500.070.8202061
ATAGGGC10350.070.3803563
CGTACAC16300.070.349724
ATAGGGA18300.069.851853
TCCGTAC16500.069.244392
TAGAGGG29100.068.991752
ATCCGTA16850.068.710731
TAGGGCG3300.068.35774
AGTAGGG25450.067.4322052
GTAGGGA12950.067.137023
TAGACGG2750.066.745391
ACGGGAT6000.066.577544
GGATAGG12600.066.487321
TATAGGG13750.066.337842
GATAGGG24900.065.7116242
AGGGAAT21350.065.3760455
Produced by FastQC (version 0.11.5)