FastQCFastQC Report
Mon 27 Feb 2023
SRR3128421.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128421.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1846843
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142560.7719118517383449No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACGAGTCT40960.2217838765937332No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC37370.20234529951923363No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG35710.19335698811431185No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG35490.19216576612088845No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA28240.1529095867921637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA1750.083.2480244
CGTTTTT53150.081.031571
AGTAGGG25950.072.620182
CCGTACA19950.072.3178943
ATCCGTA20900.071.5391
CGTACAC20200.071.4228744
TAGGGCG4050.070.782374
TCCGTAC21050.070.771352
ATAGGGC11350.069.974723
ACGGGAT5750.069.470664
GTAGGGC8400.069.373363
GTAGGGA13700.068.948663
AAGGGAT28200.068.159214
GGTAAGG9900.067.914561
TAGGGCA8250.067.216894
AAGGGCG8700.066.981174
AGGGAAT19750.066.627425
TATAGGG12600.066.016592
GAATAGG10500.065.824881
GGATAGG12400.065.597381
Produced by FastQC (version 0.11.5)