FastQCFastQC Report
Mon 27 Feb 2023
SRR3128420.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128420.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2051607
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175770.856743031194571No Hit
CCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG41050.20008705370960422No Hit
CTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTGC40890.1993071772517836No Hit
GCTGTCTCTTATACACATCTGACGCACGAGTCTTCGTATGCCGTCTTCTG39430.1921908045741704No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCACGAGTCT22390.10913396181627377No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT61950.081.6445161
ACGGGTA2200.081.179114
GTAGGGA16700.072.608353
TAGGGCG3850.072.023564
GTAGGGC10300.070.725793
AGTAGGG28650.070.7130362
CGTAGGG6600.070.507962
ACGGGAT5800.070.497644
TAGAGGG35500.069.912112
ATAGAGG14150.069.4943851
GAATAGG12500.068.881241
AAGGGCG9800.067.6201864
TAGGGAT12950.067.140614
TAAGGGA19550.066.831523
CGGGTAT3100.066.707455
TAAGCGG3950.066.7038351
TAGGGCA10150.066.6775744
AGGGTAT11500.066.6151665
AAGGGTA12500.066.5497744
ATAGGGC13850.066.5104143
Produced by FastQC (version 0.11.5)