FastQCFastQC Report
Mon 27 Feb 2023
SRR3128419.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128419.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1327418
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116400.8768903239220802No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG24140.18185680772748297No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA19100.1438883607123001No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATTAGACG17340.1306295379450934No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC16460.12400012656149004No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC15960.12023341554807905No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG15050.11337800150367104No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT45050.082.328291
AGGGATG29950.077.0484545
GAATAGG7500.075.30741
AGTAGGG21650.073.634762
TAGGGTA4700.072.9968644
CTACGAC3350.072.9520955
CGTACAC9250.071.6401754
GGTAAGG9100.070.85931
CGTAGGG4400.070.539492
GATAGGG20950.070.4833762
TATAGGG10150.069.960352
GTAGGGT5250.069.825573
GAGGGAT21550.069.788194
ATAGGGA13750.069.727923
TAGGGCA6950.069.651694
GTAAGGG21300.069.3251952
GTAGGGC8050.068.891453
GGATGGC24450.068.815057
TAGAGGG23150.068.660472
AGTAAGG8200.068.304731
Produced by FastQC (version 0.11.5)