FastQCFastQC Report
Mon 27 Feb 2023
SRR3128418.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128418.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273173
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108880.8551862158559755No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG27170.21340383435715338No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCATTAGACG22320.1753100324936203No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA22110.17366061014488998No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC19040.14954762628488039No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG17260.1355668082813569No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC14380.11294615892734138No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT41850.083.7239761
GGTAAGG9100.075.457171
AGGGATG24250.074.9975365
ACGGGTA1200.074.405164
CGTAGGG4300.073.221242
AGGGCAT9650.073.048575
AGTAAGG8500.073.0371861
GAATAGG7850.072.494451
AGTAGGG21700.071.680172
CCGTACA10500.071.160423
GAGGGAT17850.071.081454
GATTAGG3200.070.547291
CGTACAC10550.069.932314
ATAGGGA11650.069.78323
TATAGGG9950.069.426462
AGGGAAT15100.069.402595
ATAGAGG8750.068.80041
ATAGCGG2400.068.587641
GGATAGG8450.068.460081
ATCCGTA11350.068.371821
Produced by FastQC (version 0.11.5)