FastQCFastQC Report
Mon 27 Feb 2023
SRR3128417.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128417.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1404028
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129320.9210642522798691No Hit
GCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG28350.20191905004743496No Hit
CTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTGC20570.14650704971695722No Hit
CCTGTCTCTTATACACATCTGACGCATTAGACGTCGTATGCCGTCTTCTG18850.13425658177757138No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC16770.11944206240901177No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT50650.082.9409261
AGGGATG30700.077.768635
GTAGGGC6800.075.334983
TATAGGG10400.073.670782
TAGGGCA7450.073.178054
AGGGCAT11700.070.69785
AGGGTAC7000.070.4969565
ATAGGGA13800.070.496953
AGGGAAT16550.070.141985
AGTAGGG24200.070.118532
GATAGGG20600.069.594292
TAGGGAT9800.069.537824
AAGGGAT24950.069.5080264
TGTAGGG10350.069.485162
AAGGGCA20500.068.777514
ATAGCGG2600.068.755451
CGTAGGG4650.068.738012
TAAGGGT6950.068.299213
TAGAGGG25250.068.13352
AAGGGTA8700.068.0660254
Produced by FastQC (version 0.11.5)