FastQCFastQC Report
Mon 27 Feb 2023
SRR3128416.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128416.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11107426
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4271893.8459765565847572No Hit
CGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT220670.19866889052423128No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT181320.16324214088844705No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCT179460.16156758550540873No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG168500.15170031292578498No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT162800.1465686109455062No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT118210.10642429668223763No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1299750.089.245911
ATAGGGC50700.072.727783
TAGTAGG40900.070.6236651
GGTAAGG63000.068.362621
AGTAGGG171350.068.185672
GAATAGG63500.067.8243261
TAGGGCA39150.067.6006854
ATAGAGG74350.067.243061
GTTTTTT1744000.067.182222
GGATAGG59400.067.032171
TAGAGGG203350.066.394782
AGTAAGG62100.066.014711
AAGGGGC394350.065.953784
TATAGGG78650.065.8035662
AGATAGG59600.065.621311
AATAGGG145700.064.869162
ATAGGGA59950.064.726863
ATAAGGG134400.064.121292
GATAGGG156350.064.034772
AGGGGCT481250.063.4155355
Produced by FastQC (version 0.11.5)