FastQCFastQC Report
Mon 27 Feb 2023
SRR3128414.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128414.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11143323
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4029453.616021899392129No Hit
CGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT232150.2083310337499864No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT183830.16496874406314885No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCT180390.16188169363842367No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG172240.15456789684728695No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT164190.1473438398940783No Hit
CTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTGC116180.10425974370481766No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT114710.10294056808727522No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1277500.089.112131
ATAGGGC46900.069.142043
AGATAGG57250.067.973241
AGTAGGG169200.067.887812
TAGTAGG39300.067.409931
GGATAGG59000.067.153411
GTTTTTT1717800.067.081672
ATAGAGG75900.066.832081
GGTAAGG63100.066.518661
GAATAGG59600.066.477371
AAGGGGC410150.066.160694
TAGGGCA36450.066.143294
AATAGGG139650.065.418492
ATAGGGA58700.065.010553
TAGAGGG214400.064.803932
GATAGGG156300.064.1653442
GTATAGG37250.063.666691
AGGGGCT484000.063.493895
AGTAAGG66850.063.2095871
TATAGGG81400.063.191922