FastQCFastQC Report
Mon 27 Feb 2023
SRR3128413.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128413.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13334314
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900723.675269683914748No Hit
CGCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCT220710.16552032598002417No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT200160.1501089594860298No Hit
CGTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTT194400.14578927719866203No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCT190910.14317196970162843No Hit
CCTGTCTCTTATACACATCTGACGCCACGGTGTTCGTATGCCGTCTTCTG188270.14119211531991824No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT143050.10727960958471505No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1537050.088.881351
ATAGGGC56750.071.248423
AGATAGG67650.069.4127961
GGTAAGG74250.068.692541
ATAGAGG90200.068.647721
AGTAGGG199500.068.4429862
TAGGGCA44400.068.0882644
TAGTAGG43950.067.446341
GTTTTTT2059350.067.080592
GAATAGG71200.066.8770751
ATAGGGA73200.066.477073
AAGGGGC477700.066.267054
GGATAGG74200.065.758461
TAGAGGG256050.065.5385742
TATAGGG99550.065.132572
AATAGGG168600.064.867532
GATAGGG190100.064.778162
ATAAGGG169000.064.4914252
AGTAAGG79350.063.9810371
AGGGGCT579700.063.779275
Produced by FastQC (version 0.11.5)