FastQCFastQC Report
Mon 27 Feb 2023
SRR3128412.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128412.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10296191
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4214304.093067038092048No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT259510.25204466389560953No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT197730.19204189199675878No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCT195530.18990517949793279No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT191490.185981398363725No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT117120.11375080357386533No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG114350.11106048829125258No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1218650.089.429131
GGTAAGG59250.069.365251
ATAGGGC44400.069.2704543
GGATAGG52600.068.367961
GTTTTTT1609500.068.25462
AGTAGGG151650.067.644942
TAGGGCA34850.067.574844
ATAGAGG66950.067.371341
AGTAAGG60550.066.8640751
AGATAGG52200.066.363711
TAGAGGG186500.065.8893362
AAGGGGC365800.065.8596954
GAATAGG55450.065.618991
TAGTAGG37500.065.230381
AATAGGG129300.064.803342
ATAGGGA51900.063.7070583
TATAGGG67800.063.5640142
GATAGGG142200.063.5285452
AGGGGCT442100.063.524445
AGGGACT155500.062.770695
Produced by FastQC (version 0.11.5)