FastQCFastQC Report
Mon 27 Feb 2023
SRR3128411.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128411.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7053720
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2564633.6358545561774496No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT256700.3639214485406282No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT205300.29105209733303844No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCT194840.2762230425931282No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT115670.16398439405023163No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG110800.15708023567706117No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC96770.1371900217190362No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTC95420.13527613798109367No Hit
CGTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTC84050.11915698383264434No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT774450.089.767891
GGTAAGG40000.072.069631
ATAGGGC34150.070.736573
AGATAGG35850.070.3116761
GGATAGG35100.069.000451
GTTTTTT1019350.068.62832
AGTAAGG39850.067.738471
ATAGAGG43300.067.228681
TAGGGCA22650.067.227724
ATAGGGA33050.066.976283
TAGTAGG22750.066.76871
GAATAGG38300.066.550661
AGTAGGG104150.066.243692
AATAGGG84550.066.147152
AAGGGGC251500.066.020264
TATAGGG46800.065.9777152
GATAGGG93300.065.232772
ATAAGGG82500.064.8285142
AGGGACT98900.064.673635
GTATAGG22000.063.9146351