FastQCFastQC Report
Mon 27 Feb 2023
SRR3128410.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128410.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10273575
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3938843.8339526406338593No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT266140.25905295868283434No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT195790.19057630863647756No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCT192930.1877924675684949No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT192480.1873544506172389No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT118260.11511085479008037No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG112030.10904675344269156No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1178950.089.425861
ATAGGGC44750.068.999893
ATAGAGG65150.068.106961
GTTTTTT1568850.067.887762
GGATAGG54100.067.7539141
GGTAAGG57850.067.5101241
AGTAGGG153400.067.339682
AGATAGG53800.067.1696551
TATAGGG69400.065.988132
AAGGGGC353600.065.698344
TAGGGCA37000.065.669684
ATAGGGA53500.065.269623
GAATAGG54800.065.257021
AGTAAGG59400.064.797921
TAGTAGG35700.064.715381
AGGGGCT444800.064.0828865
AATAGGG125200.063.6173252
GATAGGG141850.063.608032
GTATAGG33000.062.8809131
AGGGACT153700.062.2863965
Produced by FastQC (version 0.11.5)