FastQCFastQC Report
Mon 27 Feb 2023
SRR3128409.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128409.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12419940
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4899993.9452606051236963No Hit
CGCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCT268240.21597527846350306No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT232590.18727143609389416No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCT227820.18343083783013445No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTT225490.1815548223260338No Hit
GAGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT145770.11736771675225485No Hit
CCTGTCTCTTATACACATCTGACGCACAGCGAATCGTATGCCGTCTTCTG141750.11413098614002967No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT1413400.089.118581
ATAGGGC55900.069.807943
ATAGAGG85650.068.340471
GTTTTTT1874000.067.837482
AGTAGGG182700.067.142932
GGATAGG68550.066.924041
AGATAGG65550.066.900321
TAGAGGG228400.066.759892
AAGGGGC435400.066.658434
GGTAAGG67700.066.513261
GAATAGG73050.065.828761
TAGTAGG42500.064.988151
AATAGGG163000.064.844542
TATAGGG86450.064.775612
ATAAGGG152000.064.309582
AGGGGCT531950.064.237135
AGGGACT181850.063.756115
AGTAAGG71750.063.742541
GATAGGG178000.063.342312
ATAGGGA67450.063.2213633
Produced by FastQC (version 0.11.5)