FastQCFastQC Report
Mon 27 Feb 2023
SRR3128408.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128408.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences912090
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127871.4019449834994353No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCCAAATGC88590.9712857283820675No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG46570.5105855781775921No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC41710.4573013628041092Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG36550.40072799833349776No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATG30680.3363703143330154No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA27530.3018342488131654No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCCCAAATGCTC21840.2394500542709601No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT18640.204365797234922No Hit
CGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT14590.15996228442368626No Hit
ACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG14520.15919481630102292No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGT12300.13485511298227149No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGC11610.12729007005887577No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG11240.12323345283908385No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC11150.1222467081099453No Hit
AGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT11120.12191779320023244No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG10980.12038285695490578No Hit
GGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT9750.1068973456566786No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG9140.10020940915918386No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGG850.088.564251
CGTTTTT51500.087.6130451
TACGGGT550.085.441833
TAGGGCA3450.083.089094
AAGGGTA4300.078.685974
ATAGGGC4550.078.493823
ATAGGGA6550.076.049763
GAATAGG4500.075.279611
GTAGGGT2750.075.188813
ATCCGTA35450.075.1203461
CCGTACA33950.075.022723
CGTACAC33900.074.856124
TCCGTAC35500.074.659322
ATAGGGT2650.074.479493
AGTAGGG11500.074.371552
TAGGGTA2200.072.6255654
AGGGTAT3700.072.394645
TAGGGAT3700.072.394644
GTACACC34900.072.30735
AGGGATG7700.072.015275
Produced by FastQC (version 0.11.5)