FastQCFastQC Report
Mon 27 Feb 2023
SRR3128407.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128407.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995131
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154891.5564784937862453No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG53380.5364117890006441No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCCAAATGC50580.5082747899522776No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC46150.4637580378864692Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG39070.39261162600702826No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT22370.2247945245399852No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA18600.186910065107006No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATG16960.1704298228072485No Hit
CGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT15600.15676328041232762No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCCCAAATGCTC12230.12289839227197223No Hit
AGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT12230.12289839227197223No Hit
ACTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG12170.12229545657807867No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG11470.11526120681598705No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG10470.10521227858442757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64550.086.901611
TACGGGT650.079.536513
TAGGGCA5600.078.8909154
AAGGGTA5750.077.650274
GGTAAGG4650.076.8500751
CGTAGGG1800.073.1093142
GTAGGGA7300.072.107823
AGGGCAT9850.072.0489965
AGGGTAA4700.071.998235
GAATAGG4900.071.969551
AGTAGGG15200.071.425872
TACGGGG3950.071.390653
ATAGGGT3700.071.1333853
GATTAGG2450.071.009961
ATAGGGC5700.070.910543
AATAGGG13500.070.3241962
ATAGGGA8650.070.090763
ATAGAGG5450.069.8830951
AGTAAGG5250.069.858451
AGGGTAC3850.069.582715