FastQCFastQC Report
Mon 27 Feb 2023
SRR3128406.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128406.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences859200
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135361.5754189944134078No Hit
CCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG43430.5054702048417132No Hit
CTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTGC36530.42516294227188084Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCTG32620.3796554934823091No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCCAAATGC26730.31110335195530725No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT19340.2250931098696462No Hit
CGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT13490.15700651769087523No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG11930.13885009310986965No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG11630.1353584729981378No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA10490.1220903165735568No Hit
AGCTGTCTCTTATACACATCTGACGCCCAAATGCTCGTATGCCGTCTTCT10030.1167364990689013No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG8950.10416666666666667No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT500.094.002515
CGTTTTT53950.086.923321
TAGGGCA3750.079.077164
GATTAGG2200.077.045661
GAATAGG4300.076.647491
GGTAAGG3950.075.095141
AGTAGGG11600.075.0681152
AAGGGTA4350.073.580284
AAGGGTC5500.072.744144
AGGGTAC3750.072.695275
ATAGCGG1950.072.436091
AGGGTAA3400.071.884265
TAGCGGG3550.071.599042
CTAGCGG1000.070.625191
ATAGGGC4200.069.4838943
ATAGGGA5200.068.794233
ATAGAGG5550.068.716411
GCATAGG2750.068.485031
TAAGGGT3000.067.466623
TAGTCGG353.5996636E-667.2620851
Produced by FastQC (version 0.11.5)