FastQCFastQC Report
Mon 27 Feb 2023
SRR3128404.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128404.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2030902
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276521.3615624978457848No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC67670.3332017005251854Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG66620.3280315839956827TruSeq Adapter, Index 23 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG57670.28396249548230296TruSeq Adapter, Index 23 (95% over 23bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTCTCTGC45340.2232505556644289No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT42670.2101036879179793No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA24180.1190603977936897No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT94400.084.7841951
ACGGGTA1400.080.572344
GAATAGG9950.077.9808961
GTAGGGA13400.072.957853
GTACGGG3850.072.0285642
ATAGAGG13250.070.98091
TAGACGG2650.070.9808961
ATAGGGC9600.070.502533
AGTAGGG30750.070.464312
TAGGGTA6150.069.54554
CGTAGGG4150.069.0868152
ATAGGGA15000.068.935813
GTAGGGC10200.068.6593253
TAAGGGA15000.068.622473
AGGGTAC9800.068.1028065
AGGGATA19000.068.0270845
ATAAGGG24300.067.311062
TATAGGG12650.067.251432
GAGGGAT22100.066.991714
GTAGGGT7450.066.8748863
Produced by FastQC (version 0.11.5)