FastQCFastQC Report
Mon 27 Feb 2023
SRR3128403.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128403.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1791099
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT244151.3631295645857655No Hit
CTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTGC56190.31371800218748375Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG55190.30813483788444973TruSeq Adapter, Index 23 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTTCTCTGCTCGTATGCCGTCTTCTG48120.26866186626199895TruSeq Adapter, Index 23 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT37600.20992697779408065No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTCTCTGC25460.14214736315524715No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT80700.085.8295751
TAGACGG2300.075.748091
GTAGGGC7300.074.795433
TAGGGCA8150.073.925164
AGGGTAC7900.073.1749955
CGTAGGG4450.072.984222
AGTAGGG26000.072.414672
GAATAGG10800.072.373931
ATAGGGC9100.070.345693
GGATAGG9200.070.118171
CGAAGGG16550.069.9643862
ATAGGGA12850.069.963283
ACGGGTA1750.069.931884
AGGGCAT15600.069.594045
AAGGGTA11000.068.8926854
AGAGGGC23150.068.723563
AGGGATG21000.067.812235
TAAGGGA12650.067.72063
ACGGGAT5700.067.7140664
AGTACGG2100.067.266651
Produced by FastQC (version 0.11.5)