FastQCFastQC Report
Mon 27 Feb 2023
SRR3128402.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128402.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2131967
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241381.1321938848021569No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTACAACGT45140.21172935603599868No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC42400.19887737474360534No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG41890.19648521764173646No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG41020.19240447905619554No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29360.13771320100170406No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27660.1297393439954746No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA27290.12800385747058937No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81050.085.177681
AGTACGG2850.075.9559251
TAGGGCA9850.074.431394
TAGGGTA6550.073.903274
CCGTACA19900.073.447173
TCCGTAC21250.071.6562042
GTACGGG4550.071.269782
ATCCGTA21850.070.643321
ATAGGGC12450.070.589483
AGGGATG29300.070.414925
CGTACAC20950.069.7660454
AGTAGGG36400.069.591332
AGGGTAC7850.069.447415
ATAGGGA20000.069.320193
GTAGGGT8000.069.320193
CGTAGGG5800.068.8745352
ATAACGG2050.068.867781
AGAGGGC28000.068.816653
GAATAGG12300.067.719981
ACGGGAT6400.067.5578164