FastQCFastQC Report
Mon 27 Feb 2023
SRR3128401.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128401.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2364603
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293191.2399121543870155No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC49560.2095912083339148No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG46240.19555079647619494No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG45640.19301337264648655No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT33290.14078473215165507No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33270.14070015135733144No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTACAACGT25840.10927838626610895No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT93000.085.596571
AGGGATG35350.073.257425
AGTAGGG37650.070.0305252
ATAGAGG16200.069.659221
AGATAGG14650.069.326371
AGAGGGC30850.069.317953
ACGGGAT6650.069.261824
CGTAGGG6350.068.833312
GAGGGAT31350.068.8120654
GTACGGG4350.068.067652
TAAACGG2150.067.796241
ATAGGGA21500.067.7661363
TAAGGGA16950.067.656513
AGTACGG2850.067.642771
AAGGGAC23000.067.433484
TAGAGGG47400.067.028242
AAGAGGG77400.066.976742
TAGGGCA12500.066.9267044
GTAGGGT7750.066.708393
AGGGAAT26000.066.521795
Produced by FastQC (version 0.11.5)