FastQCFastQC Report
Mon 27 Feb 2023
SRR3128400.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128400.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2059850
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256721.2463043425492146No Hit
CCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG38400.18642134135980773No Hit
CTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTGC38370.1862756996868704No Hit
GCTGTCTCTTATACACATCTGACGCTACAACGTTCGTATGCCGTCTTCTG37860.18379979124693546No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT29920.1452532951428502No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC27200.13204845012986383No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81800.084.871471
ACGGGTA1650.076.999284
TAGGGCA8700.074.098294
AGGGATG33000.072.923435
ACTAGGG9250.071.7272642
AGTAGGG31100.070.960942
ATAGGGA19700.070.702113
ACGGGAT7000.070.582684
GAGGGTA11000.069.299364
ATAGGGC12450.069.165363
GTACGGG3850.068.443812
GAGGGAT26350.068.39514
AGGGAAT20550.068.386515
AGAGGGC25250.068.206633
GTAGGGA13400.068.124583
AGATAGG11500.067.941211
TAAGGGA16450.067.79373
TAGGGTA6250.067.759374
AAGAGGG69600.067.743152
AGACGGG11650.067.452412
Produced by FastQC (version 0.11.5)