FastQCFastQC Report
Mon 27 Feb 2023
SRR3128399.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128399.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2668590
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT351781.3182242307735546No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG42830.1604967417250308TruSeq Adapter, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC40110.1503040931727991TruSeq Adapter, Index 27 (100% over 24bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCACCTTTC35110.13156760686354965No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33820.12673359339576332No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG33540.12568435016244534TruSeq Adapter, Index 27 (95% over 24bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA29950.11223155299240421No Hit
CGCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCT27080.10147680985089505TruSeq Adapter, Index 27 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT117650.085.947381
TCCGTAC19600.074.567052
CCGTACA19100.073.81263
TAGGGCA12900.072.858954
CGTACAC19150.072.3928764
AGGGATG50700.071.37165
TACGGGT1600.070.4910353
GAATAGG16350.069.6443251
ATAGCGG6450.069.3031
AAGGGAT45850.069.286714
AAGGGTA18600.068.97514
ATCCGTA21650.068.895291
GGTAAGG16900.068.76991
TAGCGGG15200.068.636012
GTACGGG7400.068.585882
AGGGCAT23350.067.824355
TAAGGGA23850.067.5846943
AGTAGGG42000.066.574872
TGTACGG2550.066.4278951
CGTAGGG8450.066.180932
Produced by FastQC (version 0.11.5)