FastQCFastQC Report
Mon 27 Feb 2023
SRR3128398.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128398.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2978500
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT423441.421655195568239No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG46220.15517878126573778TruSeq Adapter, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC44230.14849756588887025TruSeq Adapter, Index 27 (100% over 24bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC39560.13281853281853281No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG35200.11818029209333557TruSeq Adapter, Index 27 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT140850.086.201551
TAGGGCA16300.074.6795654
AGGGATG53900.072.983785
ACGGGTA3600.071.804194
GTAGGGC12450.070.215543
GAATAGG17750.069.674381
ATAGGGC15800.069.606283
TAGAGGG56800.068.6782762
AAGGGAT55650.068.23954
AGTAGGG50150.068.132312
AGTAAGG18000.067.922951
AGATAGG17150.067.1765441
TAAGGGA28200.066.998733
TAGGGAT23500.066.9987264
AAGAGGG103300.066.654112
CGTAGGG8400.066.582072
AGGGCAT26800.066.465165
ATAGGGA32150.066.368893
CGAAGGG30050.066.0020752
AGGGTAC14050.065.89915
Produced by FastQC (version 0.11.5)