FastQCFastQC Report
Mon 27 Feb 2023
SRR3128397.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128397.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2617816
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT370331.4146525195048085No Hit
CCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG38330.14641976365031TruSeq Adapter, Index 27 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTGC35510.13564742518190737TruSeq Adapter, Index 27 (100% over 24bp)
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC33450.127778270130521No Hit
GCTGTCTCTTATACACATCTGACGCCACCTTTCTCGTATGCCGTCTTCTG30500.11650933449868135TruSeq Adapter, Index 27 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT127150.085.7408451
AGGGATG47050.073.6208655
GAATAGG14950.071.474361
TAGGGCA13800.071.268864
ACGGGTA3300.069.873864
AAGGGTA18200.069.552624
GGTAAGG16950.068.872771
AAGGGAT49550.068.758684
ATAGGGA25900.068.6791153
AGTAGGG42750.068.35782
GAGGGAT38250.067.7879944
AGATAGG15950.067.2883151
TATAGGG22750.067.2255862
TAGAGGG47300.067.154522
TAAGGGA24000.067.057523
ATGCGGG12800.066.910472
ATAGCGG5450.066.505841
AGGGCAT25950.066.107185
GTACGGG6700.065.319192
GTAGGGC10950.064.892653
Produced by FastQC (version 0.11.5)