FastQCFastQC Report
Mon 27 Feb 2023
SRR3128396.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128396.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909022
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227371.1910287047503905No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGACCTTA86830.4548402270901016No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG55130.2887866142977923TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC52640.2757432863529074TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG50690.26552863193823856TruSeq Adapter, Index 23 (95% over 21bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA46770.2449945574225965No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATG23390.12252347013287433No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21660.11346123826755271No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20100.10528951473581762No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT84650.085.825671
AGTACGG2550.077.500761
GTACGGG4250.076.299572
TCCGTAC36250.075.582712
CCGTACA34950.075.570283
GAGGGTA11650.075.435824
AGTAAGG10350.075.013681
ATCCGTA36950.074.75161
CGAAGGG15800.074.658382
CGTACAC35300.074.554734
GGTAAGG11800.073.372391
AGGGCAT18800.072.993965
ACGGGTA2550.071.8763964
AGGGTAT11750.071.594085
AGGGTAC9650.071.589935
TAGGGTA8450.069.5208744
AGAGGGC25350.069.5208743
TACGGGT2300.069.472513
TAGAAGG14550.069.206621
TAGGGCG2600.068.686624
Produced by FastQC (version 0.11.5)