FastQCFastQC Report
Mon 27 Feb 2023
SRR3128394.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128394.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830465
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242181.3230517928504504No Hit
CCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG47840.26135435531408685TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTGC47400.2589505945210643TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGACCTTATCGTATGCCGTCTTCTG44680.24409098234601592TruSeq Adapter, Index 23 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGACCTTA27230.1487600145318266No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC21040.11494347064816862No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT20280.11079152018749333No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT85700.087.396661
TACGGGT1850.078.84853
TAGGGCG2150.078.789354
CGTAGGG6750.078.0760352
CGAAGGG14350.074.435042
ACGGGTA2150.074.412174
GTACGGG3050.072.5106052
ATAGCGG2750.071.898871
AGTAAGG12250.071.479631
GGTAAGG10700.070.8349461
AGGGCAT16350.070.718675
AGTACGG2200.070.614961
AAGGGCA30650.069.699374
AGGGATG24750.068.746435
AAGGGTA12350.068.581834
AGGGTAT11300.068.215365
AGAGGGC25250.068.206093
TAGGGCA10250.067.942474
TAGAGGG32050.067.8293152
TAAGGGA16250.067.469283
Produced by FastQC (version 0.11.5)