FastQCFastQC Report
Mon 27 Feb 2023
SRR3128393.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128393.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2304888
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT295611.2825352034458941No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTAGCGGT74300.3223583965902031No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC52120.2261281242298975No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG50880.22074825327738265No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA46870.2033504447938468No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG44330.19233038655240514No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34820.1510702472311019No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT92800.085.883311
CGTAGGG6600.079.751092
CCGTACA33550.077.743313
TCCGTAC34750.077.628232
CGTACAC33450.077.413744
ATCCGTA35200.076.9880141
TAGACGG3550.075.542241
GGTAAGG12750.071.956121
AAGGGTA16550.071.275184
GTACACC36550.070.719295
ATAGAGG14700.070.092271
TACACCC35450.069.997136
ATAGGGA18000.069.449923
AGGGATG28000.069.319375
AGGGTAC10350.069.018545
AGAGGGC35000.068.882973
AGTAGGG36400.068.686722
TAGGGCA13100.068.521164
ACGGGTA2900.068.063444
GTAAAGG18050.067.770311
Produced by FastQC (version 0.11.5)