FastQCFastQC Report
Mon 27 Feb 2023
SRR3128392.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128392.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2553099
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT352051.378912451103541No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC55880.21887126194479728No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG54940.21518946190492416No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG47660.18667509563867285No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTAGCGGT42280.16560266562322887No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT38360.1502487760952474No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA34060.13340649931710444No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT110600.086.495911
AGTAGGG38700.073.238982
AGAGGGC36200.071.155423
ATAGGGA20100.069.921563
ATAGAGG16900.069.853271
AGTACGG3250.069.463651
AGGGCAT21500.069.083785
TAGAGGG48350.068.829212
AAGGGCG9300.068.735864
AAGGGTA18050.068.4866644
AGGGTAA11850.068.224065
AGGGTAC12300.068.02095
GTAGGGA18000.067.8948443
TAGGGCA14550.067.839674
CGTAGGG7350.067.788262
TAGGGTA10350.067.666454
AGGGATG32700.067.270645
ATAGGGC16500.067.230143
AGACGGG18200.067.148752
AAGGGAT40450.067.047834
Produced by FastQC (version 0.11.5)