FastQCFastQC Report
Mon 27 Feb 2023
SRR3128391.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128391.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2235251
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT315141.4098640376405156No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC47220.2112514433502099No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG47190.21111723023499374No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTG40620.1817245580026583No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34560.15461350872899735No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCTTAGCGGT23130.10347831183164664No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99300.085.747731
AGTAGGG34250.074.774932
CGTAGGG6400.074.2949142
TAGACGG3450.072.348621
TAGGGTA8200.072.339324
TAGGGCA14650.072.304054
ATAGGGC15400.072.145463
AGGGCAT20800.071.857435
AGGGTAC11500.071.1146855
AGTAAGG14450.071.0496751
AGAGGGC32050.070.213013
ATAGAGG15050.070.0946661
ATAGCGG4650.069.882681
AGGGTAA10100.069.3382345
AGGGAAT23750.069.2647555
AGATAGG11400.068.576711
GTAGGGC10300.068.560153
ATAGGGA18550.068.2693943
GTAGGGA13800.068.228943
AGGGATG27200.068.082465
Produced by FastQC (version 0.11.5)