FastQCFastQC Report
Mon 27 Feb 2023
SRR3128390.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128390.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2637413
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316421.1997362567030647No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGATAAGTG66240.25115520398208396No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG65760.24933523873583696TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC63250.23981833713567044TruSeq Adapter, Index 21 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG59600.22597901807566734TruSeq Adapter, Index 15 (95% over 21bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA45590.17285878245083344No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT44120.16728513888420204No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT104000.084.964961
CCGTACA30650.074.67333
TCCGTAC32350.074.090542
AGGGTAC11900.073.456945
ATCCGTA33350.072.376761
ATAGCGG5400.072.320591
AGTAGGG39300.072.109362
CGTACAC31500.072.061524
TAGGGCA14550.071.383224
GAATAGG14250.070.660481
ATAGGGC15950.069.537363
GTAGGGA17350.069.343753
AAGGGAT38200.069.264694
ATAGGGA22950.069.21513
AGGGCAT24150.069.084095
CGTAGGG6950.068.973492
GTAGGGT11150.068.703623
AGGGATG36850.068.2312245
GGTAAGG14650.068.088831
AGTAAGG15900.068.062561
Produced by FastQC (version 0.11.5)