FastQCFastQC Report
Mon 27 Feb 2023
SRR3128389.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128389.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2930415
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT392711.3401173553916426No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG75840.2588029340554153TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC72800.24842897678315187TruSeq Adapter, Index 21 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG64390.2197299699871861TruSeq Adapter, Index 15 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT50210.17134091928958867No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGATAAGTG40320.13759143329528412No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA30420.10380782244153132No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT117200.086.385531
AGTAGGG44400.072.722462
CGTAGGG7700.072.025472
ACGGGTA3600.071.8050464
ATAGCGG5450.071.615361
TAGGGTA9850.071.5731054
AGGGTAC12750.070.038725
AAGGGAT42950.069.596714
ACTAGGG13450.069.5385362
GAATAGG16300.069.5270841
TAAGGGA24000.069.520343
AGGGATG39550.069.400275
TAGCGGG14600.069.2118452
TAGGGCA15050.068.7038354
AGAGGGC38750.068.164683
ATAGAGG18450.068.051781
ATAGGGC16650.068.029543
ATAAGGG44000.067.829062
TATAGGG22000.067.508612
AGGGCAT26850.067.21745
Produced by FastQC (version 0.11.5)