FastQCFastQC Report
Mon 27 Feb 2023
SRR3128388.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128388.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2554036
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT336871.3189712282833916No Hit
CCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG60040.23507891039907033TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTGC55300.2165200490517753TruSeq Adapter, Index 21 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGATAAGTGTCGTATGCCGTCTTCTG52010.20363847651325198TruSeq Adapter, Index 15 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT43870.1717673517522854No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT105400.085.398271
TAGGGCA14050.076.0427254
CGTAGGG6350.074.86252
TAGCGGG12900.072.242392
ATAGCGG5900.071.8112261
AGGGCAT24800.071.452795
AGTAGGG40650.071.092612
GTAGGGC10450.070.711743
GTAGGGT11600.070.599143
GAATAGG15550.070.538681
AGGGTAC13200.070.505145
AGGGATG36600.069.734595
GGTAAGG16100.068.7137761
GTAGGGA17850.068.555663
AGTAAGG14200.068.293711
TAGAGGG43200.068.203592
ATAGGGC14900.067.598293
TAGGGAT19150.067.5883944
TAGGGTA10800.067.548564
AAGGGAC25300.067.529614
Produced by FastQC (version 0.11.5)