FastQCFastQC Report
Mon 27 Feb 2023
SRR3128387.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128387.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1316304
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195641.4862828039723346No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAGGCGTA120930.9187087481311309No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC69270.5262462166794297No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG65830.5001124360330136TruSeq Adapter, Index 14 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG50940.3869926703861722TruSeq Adapter, Index 14 (95% over 21bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA34890.265060350800423No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATG31670.24059791659069638No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27070.20565158200537265No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGAGGCGTATC24790.18833035529786432No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT20700.15725850563395688No Hit
ACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG19230.14609087262516865No Hit
AGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT17420.13234024966876953No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC16420.12474322041109046No Hit
AGAGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT16170.12284396309667069No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCGAGGCGTATCGT13660.1037754196598962No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT70900.088.562741
CGTAGGG3650.079.827362
CCGTACA43850.076.7355043
CGTACAC43950.076.347054
ATCCGTA45950.076.258231
TCCGTAC45650.076.180512
AGGGTAT5050.075.378345
TAGGGCA5000.074.252324
TAGGGCG2600.074.107724
AGTAAGG7900.073.2308351
TAGGGTA4700.072.9924554
TACACCC44300.072.455266
GTAGGGC4350.072.3833163
AGGGATG13400.072.246265
AAGGGTA7000.071.835434
AGGGTAC8150.071.501815
GAGTAGG8000.071.1395951
TGTAGGG8050.070.638662
AGATAGG6500.070.1898651
GTACACC47650.070.1228565
Produced by FastQC (version 0.11.5)