FastQCFastQC Report
Mon 27 Feb 2023
SRR3128386.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128386.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468217
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241901.6475766184426417No Hit
CTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTGC72920.49665682933789757No Hit
CCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG71960.4901182863296093TruSeq Adapter, Index 14 (95% over 21bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGAGGCGTA71800.4890285291615613No Hit
GCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG57260.38999684651519495TruSeq Adapter, Index 14 (95% over 21bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT30870.21025502361027015No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA25490.17361193883465453No Hit
CGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT23370.15917265635801792No Hit
AGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCT19130.13029409140474468No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATG18480.12586695290954947No Hit
ACTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTTCTG17050.11612724822012005No Hit
AGAGCTGTCTCTTATACACATCTGACGCGAGGCGTATCGTATGCCGTCTT16360.11142767043291284No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGAGGCGTATC14940.10175607556648643No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86750.087.553791
TAGGGCA7050.078.004044
CGTAGGG3500.076.546822
GTAGGGT6150.075.662463
AGTAAGG8650.074.486281
ATAGAGG9100.074.420561
TAGGGTA5450.074.1689764
ATAGGGC8900.073.40833
AGGGATG15300.073.1124045
GAGGGAT14800.070.503654
AGGGCAT12800.070.501265
AGGGTAC8200.069.354895
AGAGGGC16900.068.973993
CCGTACA28700.068.128973
GTAGGGC6100.067.806793
AAGGGAT21300.067.745294
ATCCGTA29950.067.521721
ATAGCGG1750.067.1852341
TAACGGG4200.067.146342
AAGAGGG46750.067.060162
Produced by FastQC (version 0.11.5)