FastQCFastQC Report
Mon 27 Feb 2023
SRR3128382.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128382.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2470544
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT364221.4742502056227293No Hit
CCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG60090.24322578347117071No Hit
CTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTGC55830.22598261759353405No Hit
GCTGTCTCTTATACACATCTGACGCGCTTAACATCGTATGCCGTCTTCTG54860.22205635681857924No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC34290.13879534224041343No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT27960.11317345491519276No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT102100.086.038361
ATAGCGG3600.081.0765841
TAGGGCA11600.077.0787354
CGTAGGG6250.073.787882
AGTAGGG37100.072.807642
AAGGGTA15900.072.511674
AGGGCAT25300.072.264125
ACGGGTA2100.071.7083364
GAATAGG12400.071.374381
TAGGGCG4550.071.363594
GTAGGGT12350.071.254723
AGGGTAC13000.070.860855
TAGGGTA9450.070.7123954
GGTAAGG11200.070.615081
AGGGTAA13400.070.499325
AGAGGGC34850.070.4866263
TAGCGGG10300.070.359462
AGTAAGG15450.069.7771451
ATAGGGC14900.069.1666643
GTAGGGC9550.068.493663
Produced by FastQC (version 0.11.5)