FastQCFastQC Report
Mon 27 Feb 2023
SRR3128381.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128381.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2638663
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331421.2560148832950626No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGGAATAT82490.3126204445205773No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG70760.2681661129140023No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC66710.2528174306457475No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA59970.22727419151289877No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG59440.2252655985247074No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT51190.19399976427455873No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC30660.11619520946782518No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT106050.085.1336751
CGTAGGG6600.077.614362
CCGTACA38500.076.780233
CGTACAC38400.076.3682564
TCCGTAC40400.075.961112
TAGGGTA8500.075.7462544
ACGGGTA3300.075.478184
ATCCGTA41050.075.299011
GTAGGGT9900.075.003483
ATAGGGA22800.073.585613
AGGGTAC15400.073.240295
ATAGCGG4900.072.9717251
AGGGCAT22750.072.921035
GTAGGGA16850.072.2369463
GAATAGG14200.072.227941
AGGGATG38250.071.384555
TAGGGCA14950.071.043894
AGTAGGG39750.070.937142
GTACGGG6000.070.4937742
GTAGGGC12900.069.583013