FastQCFastQC Report
Mon 27 Feb 2023
SRR3128380.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128380.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2903394
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT402031.3846897803053944No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG78850.27157871098445474No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC76000.2617626129970648No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG64760.2230493002327621No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT57290.1973207907710769No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGGAATAT47820.16470379149367945No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA42030.14476161347719255No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT129150.085.525031
ATAGCGG6500.077.406071
TAGGGTA10900.073.738564
AGGGCAT24100.072.744795
TAGGGCA17450.072.1879044
GAATAGG17350.072.091951
GGTAAGG15900.071.8643951
AGGGATG40650.071.687145
CGTAGGG7550.071.593892
ATAGGGC17650.071.369923
AGGGTAC17400.070.231875
AGTAGGG45600.069.67982
ATAGGGA24950.069.326993
AGTAAGG17850.069.282281
AGGGTAT14050.068.912985
AAGGGTA22250.068.233494
AGAGGGC40800.068.0851443
TCCGTAC25500.067.83172
GAGGGAT30950.067.7328344
AATAGGG42150.067.6887052
Produced by FastQC (version 0.11.5)