FastQCFastQC Report
Mon 27 Feb 2023
SRR3128379.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128379.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2542624
Sequences flagged as poor quality0
Sequence length100
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT346351.3621754533898838No Hit
CCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG63610.2501746227519287No Hit
CTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTGC57540.2263016474319443No Hit
GCTGTCTCTTATACACATCTGACGCGGGAATATTCGTATGCCGTCTTCTG53290.209586631763092No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT51550.20274330770102067No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGGAATAT26720.10508828674629044No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT110900.085.256431
GAATAGG15750.076.5341
CGTAGGG5550.073.78052
AGGGTAC14850.073.1157765
GGTAAGG14500.073.065021
TAGGGTA10050.072.5907064
ATAGGGA22600.072.0581363
AGGGCAT22750.071.692485
GTAGGGC10000.071.070983
AGAGGGC36300.070.79483
AGTAAGG14450.070.710981
ACGGGTA2600.070.60034
AGTAGGG40900.070.542772
GTAGGGA17950.070.534763
AGGGATG39700.070.44535
GTAGGGT11500.069.986393
TAAGGGA19350.069.809783
ATAGCGG5150.069.486651
AGGGTAA12450.067.956145
AATAGGG34850.067.7979052
Produced by FastQC (version 0.11.5)