FastQCFastQC Report
Mon 27 Feb 2023
SRR3128378.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128378.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences650972
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTTGTCCC157102.4133142439306146No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91661.4080482724295362No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG65991.0137148756014083No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATG54710.8404355333255502No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC53080.8153960539009358No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA47170.7246087389319357No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGTTGTCCCTC38520.5917305198994735No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG32170.4941840816502092No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGC21490.33012172566562004No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGT21250.3264349311491124No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT21100.3241306845762951No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG20370.31291668458858446No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG20190.31015158870120374No Hit
ACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG19640.3017026846008738No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG16480.2531598901335234No Hit
ATCCGTACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCG13780.21168345182281265No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCG12900.1981652052622847No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGG11850.18203547925256386No Hit
ATCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC10970.1685172326920359No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC10470.16083641078264505No Hit
AGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT10170.15622791763701052No Hit
ACACCGAGATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGC9920.15238750668231507No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG9850.15131219161500034No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9100.13979095875091402No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT8700.1336463012234013No Hit
AGAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT8380.12873057520139114No Hit
GGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT8080.12412208205575663No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGG7890.1212033697301881No Hit
ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT7830.1202816711010612No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT7760.11920635603374645No Hit
ATCCGTACACCCACACACCGAATCCTGTCTCTTATACACATCTGACGCGT7580.11644126014636574No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT7260.11152553412435559No Hit
GACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC6860.10538087659684287No Hit
GAAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT6670.10246216427127433No Hit
ACACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT6550.10061876701302053No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGAT2450.090.152294
CGTAGGG950.089.041792
CGTTTTT56500.086.9289251
AGGGTAA1500.081.456755
GGATAGG3050.080.207641
CCGTACA62600.078.0735553
ATCCGTA66150.078.016961
TCCGTAC65600.077.7984542
CGGGAAT1150.077.642725
CGTACAC62650.077.4861764
TAGTAGG1700.077.4856641
TAGGGTA1400.077.204894
GTACGGG1100.076.899732
AGGGATT4100.076.7955255
GAGGGAT5450.076.742954
TACACCC59700.076.434586
GTACACC63150.076.3517465
ATAGCGG1250.075.271791
AGGGTAC2250.075.190855
AGGGTAT1750.075.190845
Produced by FastQC (version 0.11.5)