FastQCFastQC Report
Mon 27 Feb 2023
SRR3128377.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128377.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695476
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114501.646354439261743No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTTGTCCC91541.3162208329259386No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG72041.0358373258027596No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC56200.8080796461703926No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG35880.5159056531066493No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA32700.4701815734834847No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATG30020.43164681455578624No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG22870.32883952861062066No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT22460.32294428564033845No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGTTGTCCCTC21260.3056899159712197No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG20550.2954810805836578No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG18560.2668675842157026No Hit
ACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG14890.21409797031098124No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCG13200.18979806636030574No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGT11940.1716809782077311No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGC11900.17110583255209383No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG11640.16736738579045143No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC11240.16161592923407853No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGG10990.15802126888634546No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT10310.14824379274051153No Hit
AGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT10140.14579942370405305No Hit
AGAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT9240.132858646452214No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT8840.12710718989584113No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGG8820.12681961706802247No Hit
ACACCGAGATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGC8630.12408767520374535No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT8580.12336874313419874No Hit
GGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT8160.11732971375000718No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT7900.11359126698836482No Hit
ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT7850.11287233491881819No Hit
GACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC7700.11071553871017835No Hit
ATCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC7560.10870252891544782No Hit
GGAACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT7330.10539544139553342No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA7110.10223214028952832No Hit
GAAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT7020.10093806256434443No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT68050.087.6411
ACGGGTA600.086.180244
AGTCTAG452.7284841E-1183.5507059
GTACGGG1750.083.270262
GGTAAGG2100.082.870251
TAAGGGT1800.080.957213
AGTACGG1050.080.630511
GAGGGTA2000.079.912594
GAGGGAT6000.077.562224
AGGGTAC1950.077.134815
ACGGGAT1900.076.696294
GGGCGAT1850.076.222756
ACTAGGG2550.075.5805362
TAGGGTA2000.075.211854
GGGCATA1000.075.206446
AAGGGTC3200.074.918054
AAGGGTA2900.074.563484
CTAAGGG2400.074.428382
TAGGGCA2400.074.428384
TATAGGG4450.073.944232
Produced by FastQC (version 0.11.5)