FastQCFastQC Report
Mon 27 Feb 2023
SRR3128376.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128376.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences592612
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99671.68187616855548No Hit
CCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG60361.0185416427611995No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGTTGTCCC51460.8683590612407444No Hit
CTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTGC47440.8005237828461118No Hit
GCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG29330.49492754112302817No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGG22360.37731264301094136No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGG20880.352338460915405No Hit
CGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT20740.34997603828474616No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGG19050.32145822224322157No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA18210.30728368645926846No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATG16530.2789346148913623No Hit
ATCCGTACACCCACACCTGTCTCTTATACACATCTGACGCGTTGTCCCTC12200.2058682578145566No Hit
GACACACCCACACACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCG12120.20451830202560867No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGG11920.20114341255323887No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGG11820.199455967817054No Hit
ACTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCTG9700.16368213940993434No Hit
AGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT9520.16064473888480152No Hit
GACACACCCACACACCGAGATCCGTACACCCACACACCTGTCTCTTATAC9220.15558240467624687No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT9020.15220751520387707No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGG8640.14579522520637447No Hit
ACACCGAGATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGC8210.13853921284077947No Hit
AGAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT8140.13735800152545005No Hit
AAAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT7740.13060822258071048No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT7620.12858328889728862No Hit
GGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT7180.1211585320580751No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT7140.12048355416360114No Hit
ATCCGTACACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGC6880.11609619784952042No Hit
ACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTCT6820.1150837310078095No Hit
ATCCGTACACCCACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGT6350.10715274074774052No Hit
GACCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC6210.10479031811708166No Hit
ATCCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTTC6060.10225915101280433No Hit
GAAGCTGTCTCTTATACACATCTGACGCGTTGTCCCTCGTATGCCGTCTT6050.10209040653918584No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT62750.086.739421
ACGGGTA500.084.729534
CGTAGGG800.082.375932
TAGGGCA1850.081.421774
AGGGCGA1700.080.184035
TAAGAGG3000.080.0426251
AGGGTAT1500.078.340715
TAGGGTA1150.077.7710654
GTACGGG1150.077.7710652
AGACGGG3650.077.378572
ATAGCGG1100.077.046381
AAGGGTC2450.076.852174
GAGGGTA2150.076.628774
GCATAGG1300.076.05861
GCGTAGG750.075.334231
CGGGTAT507.094059E-1175.207095
AGGGCAT3250.075.2070855
CTAAGGG1900.074.324142
ATAGAGG2700.073.2416151
TAGGGTC1100.072.7475744
Produced by FastQC (version 0.11.5)