FastQCFastQC Report
Mon 27 Feb 2023
SRR3128375.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128375.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2628770
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT356501.3561475518968948No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCAGCAAGA78870.3000262480171335No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC76060.2893368381410317Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG67430.25650779642190075No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA57190.21755421737162245No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG55690.21184812669042935No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC42790.16277574683216867No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT34640.13177265413101946No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28010.10655173332014592No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT128100.087.707051
AGGGTAC12850.080.825955
CCGTACA35850.075.770533
TCCGTAC36900.075.270152
CGTACAC36450.074.265414
GAGGGTA15150.073.208414
AGGGATG36950.072.3701555
CGAAGGG25500.071.692062
TAGGGCA13050.071.664524
AGGGCAT22900.071.622855
ATCCGTA38900.071.601381
GTAGGGT9650.071.102883
TAAGGGT10150.070.378373
GTAGGGA18850.070.0578163
ATAGGGA24150.069.277873
AGTAGGG41500.068.965332
ATGCGGG10450.068.807662
TAGGGTA8950.068.787564
AGAGGGC36200.068.546773
AGGGTAA14300.068.357945