FastQCFastQC Report
Mon 27 Feb 2023
SRR3128374.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128374.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2932094
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT429641.4653009078153703No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC81010.2762871858814895Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG75140.2562673638703261No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG62140.21193044970590985No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC50280.17148154186052697No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCAGCAAGA45330.15459940915946077No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA40650.13863812006027093No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT37210.12690589046599463No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT32100.1094780726675202No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT150950.087.465531
AGGGTAC16900.077.313135
TAGGGCA15450.075.7496954
TAGGGTA10850.075.3753744
CGTAGGG6750.072.416832
GTAGGGT12500.071.441993
AGGGATG43750.071.117285
GTAGGGC13700.071.016583
CTACGAC9950.070.853835
CGAAGGG27850.070.712932
AGGGCAT27800.070.161445
GTAGGGA21200.069.615153
ACGGGTA3650.069.5361944
GAGGGTA16350.069.280224
AGTAGGG46850.069.222852
AGGGAAT28300.069.087915
TAGAGGG53250.069.023522
AAGGGTA24850.068.468714
AGAGGGC38450.068.4544453
AAGAGGG95450.068.347642
Produced by FastQC (version 0.11.5)