FastQCFastQC Report
Mon 27 Feb 2023
SRR3128373.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128373.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2531497
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371351.4669185861172263No Hit
CTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTGC65190.25751561230370806Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG59790.23618436047919472No Hit
GCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCTG50550.19968421846836082No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC41350.1633420857303011No Hit
CGCTGTCTCTTATACACATCTGACGCCAGCAAGATCGTATGCCGTCTTCT32340.1277504970379187No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT28500.11258160685159808No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT129300.087.914251
AGGGTAC12500.076.335245
CGTAGGG5550.076.327492
AGGGATG37000.074.317865
GTAGGGT10600.074.155293
AGGGCAT22050.073.118065
TAGGGCA13300.072.903284
TAGGGTA9200.072.137784
AGTAGGG40400.070.8359452
AGAGGGC35250.070.235733
AAGGGTA21150.070.10224
TAAGGGT10550.070.045253
CGAAGGG22950.069.936392
GAGGGTA13200.069.889784
ATAGCGG5200.069.728111
TAGAGGG41650.069.61412
ACGGGTA3350.068.846644
TAGGGCG4250.068.664824
AGTACGG2750.068.493261
GGTAAGG16300.068.4671
Produced by FastQC (version 0.11.5)