FastQCFastQC Report
Mon 27 Feb 2023
SRR3128372.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128372.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1960747
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262251.3375004526336136No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG77180.3936254906930879RNA PCR Primer, Index 38 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG74720.3810792519381644RNA PCR Primer, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC65590.3345153658274117RNA PCR Primer, Index 38 (95% over 23bp)
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCGACTATG61270.3124829465504729No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA39620.20206584531303629No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT35070.17886040371348266No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC26650.1359175865116713No Hit
AGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT24240.12362635260949016RNA PCR Primer, Index 38 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT23520.11995428273000035RNA PCR Primer, Index 38 (95% over 21bp)
AGAGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT23000.11730223226147994No Hit
ACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG22690.11572120217447739RNA PCR Primer, Index 27 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT22200.11322215461760238RNA PCR Primer, Index 38 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT22040.11240613908882685No Hit
GGAGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT20440.10424598380107174No Hit
ATCCGTACACCCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATG20110.10256295177297226No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT86200.086.6091461
TAGGGCA7650.079.2483444
CCGTACA29850.075.256763
AGGGCAT15000.075.193785
AGGGATG22000.075.193785
TAAGGGA14000.074.1867143
TAGCGGG9200.073.559132
CGTACAC30550.073.378544
TCCGTAC32200.072.6834262
TAGGGTA6500.072.3017044
ATCCGTA32600.072.295981
AGTTAGG4500.072.132441
CGAAGGG19050.072.036032
GGTAAGG11650.071.47291
ATAGGGA16250.071.434083
GTAGGGA14150.070.743263
GAATAGG10850.069.805591
AAGGGAT26950.069.578664
AGAGGGC25150.068.9525453
ATAGAGG11700.068.7550051
Produced by FastQC (version 0.11.5)