FastQCFastQC Report
Mon 27 Feb 2023
SRR3128371.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128371.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2158089
Sequences flagged as poor quality0
Sequence length100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT316311.4656948809803487No Hit
CCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG87330.40466357040882RNA PCR Primer, Index 38 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG81420.377278230879264RNA PCR Primer, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTGC75520.3499392286416362RNA PCR Primer, Index 38 (95% over 23bp)
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT40200.1862759135512947No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCCGACTATG34540.16004900631994323No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC32380.15004015126345577No Hit
AGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT26680.1236278948643916RNA PCR Primer, Index 38 (95% over 21bp)
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA25980.1203842844294188No Hit
CGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT25080.11621392815588236RNA PCR Primer, Index 38 (95% over 21bp)
AAAGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT24980.11575055523660052No Hit
AGAGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTT24120.11176554813077681No Hit
GGCTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCT23070.10690013247831763RNA PCR Primer, Index 38 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCGACTATGTCGTATGCCGTCTTCTG21920.1015713439065766RNA PCR Primer, Index 27 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT99050.087.5447161
CGTAGGG6150.075.6662752
TAGGGTA8000.075.2076954
GTAGGGA16700.074.8699343
AGGGATG27000.074.511335
AGGGCAT19500.073.27935
ACGGGAT4900.072.905414
TAAGGGA15300.072.811363
AGGGTAC8950.072.4766855
TAGCGGG10250.072.456192
AGTAGGG35600.072.355722
TAGACGG3550.071.5301061
GTAGGGT9650.071.116093
TAGGGCG4250.070.7837144
TAGGGCA8700.070.777354
GAATAGG12500.070.348541
ATAGGGA19550.069.72583
GACGGGT4050.069.636753
ATAGCGG4600.069.5143661
GATAGGG28400.069.514152
Produced by FastQC (version 0.11.5)