FastQCFastQC Report
Mon 27 Feb 2023
SRR3128369.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3128369.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1802571
Sequences flagged as poor quality0
Sequence length100
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225211.2493821325207162No Hit
GGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT34260.19006186164095615No Hit
CCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG32100.1780789771942409No Hit
CTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTGC31450.17447301659684972No Hit
GCTGTCTCTTATACACATCTGACGCGGCAGACTTCGTATGCCGTCTTCTG30770.17070062704880973No Hit
ATCCGTACACCCACACACCTGTCTCTTATACACATCTGACGCGGCAGACT28410.15760821626443564No Hit
ATCCGTACACCCACACACCGAATCAAAAAAAAAAAAAAAAAAAAAAAAAA23360.1295926762385504No Hit
TCCGCTACGACCAACTCATACACCTCCTATGAAAAAACTTCCTACCACTC20000.11095263376588217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT75400.084.537681
ATAGCGG3400.076.096761
CGTAGGG5550.074.516132
TAGGGCA9750.073.26554
AGTAGGG31400.070.493942
AGGGATG25650.070.173325
TAGGGTA6400.069.7596364
ATAGGGC9800.069.534843
AGGGATC13950.069.0621645
GGATAGG10400.068.753161
TAAGGGA13350.068.645793
AGGGCAT16250.068.25265
AGATAGG9150.067.863341
CCGTACA12900.067.761623
GTAGGGA15300.067.57593
AGTAAGG9550.067.483811
GTAGGGC8800.067.289673
GGTAAGG10100.067.069261
AAGAGGG59400.066.458952
TATAGGG13900.066.267692